Occludin {90000489}

Shared Reference Notes

• [1.1] 
  - high-dose #Phytosterol esters significantly increased mRNA expression level of Claudin-1 and Occludin in the colon, in comparison to the HFD group, likely enhancing intestinal barrier integrity.
• [1.2] [#Colorectal cancer] [#Carnobacterium maltaromaticum] [#Lipopolysaccharide] 
  - C. maltaromaticum could restore the gut barrier function at the early time points. - The restoration of gut barrier function was associated with increased expression of a number of associated markers, including ZO-1, occludin, and E-cadherin in mouse CRC models, enhanced thickness of colonic mucus layer, along with decreased paracellular gap and serum LPS level. - VDR signaling was activated significantly in C. maltaromaticum-treated mice, and the tumor-suppressive effect of C. maltaromaticum was dependent on VDR.
• [1.3] [#Desulfovibrio desulfuricans] 
  - Loss of Occludin and ZOs increases gut permeability. - The administration of D. desulfuricans significantly decreased the expression level of ZO-1 and Occludin in the colon of Apoe−/− mice.
• - Tight junctions, including Occludin, claudins, and ZOs, serve as barriers to the transepithelial influx of noxious substances/microorganisms from the gut lumen and are the primary control of the gut barrier. - ZO-1 is vital to tight junction assembly and maintenance as it can interact with other proteins, including claudins, Occludin, and actin. - Occludin, a transmembrane tight junction protein, can interact directly with claudins and actin.
• [1.4] [#Fiber-free diet, #Low fibre Diet] 
  - the lowest levels of tight junction proteins #ZO-1 and occludin in TG mice challenged by fiber deprivation and #Curli.
• [#Parkinson’s Disease] - In intestinal biopsies from patients with PD, especially occludin was found at lower levels, whereas #ZO-1 was unchanged.
• [1.5] [#Parabacteroides distasonis] 
  - P. distasonis is associated with increased expression of the tight junction protein occludin, indicating that this species plays a protective role in the maintenance of the intestinal epithelial
• [1.6] 
  - reduction of membrane localization of tight junction protein (#ZO-1, ZO-2, occludin, #Claudin-1, and -3) and increased permeability in colonocytes were induced by the #Ammonia treatment > mitochondrial dysfunction > increase of #Oxidative stress, > intestinal barrier dysfunction.
• - Treatment of human colon mucosal epithelial cells (NCM460) with sodium #Butyrate (SB) increased mitochondrial respiration (i.e., increased maximal respiration, spare capacity, and OxPhos-dependent ATP production) and expression of genes involved in mitochondrial bioenergetics (TFAM), complex I (NDUFA1, NDUFA4, and NDUFA6), complex IV (COX6A1), and complex V (ATP5E and ATP8). - SB treatment promoted barrier integrity by increasing tight junction gene (#Zonulin-1 and occludin) expression.
• [1.7] 
  - exposure of endothelial cells to #Hydrogen peroxide promotes a loss of occludin and cadherin. - barrier integrity was then restored upon incubation with #Vitamin D, which coincided with increased expression of VE-cadherin

References Notes